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OBJECTIVE:To observe the effects and safety of Congrong yishen granule combined with Donepezil hydrochloride tablet on mental state and quality of life in patients with Parkinson disease with dementia(PDD). METHODS:A total of 60 PDD patients were randomized into control group and treatment group,with 30 cases in each group. Both groups were given drugs for Parkinson disease. Control group was additionally given Donepezil hydrochloride tablet 5 mg,qd;treatment group was additionally given Congrong yishen granules 2 g,bid,on the basis of control group. The treatment lasted for 6 months in both groups. MMSE, MoCA,ADAS-Cog,ADL-R and TCM symptom score were observed in 2 groups before and after treatment. The occurrence of ADR were compared between 2 groups. RESULTS:Before treatment,there was no statistical significance in MMSE,MoCA, ADAS-Cog,ADL-R or TCM symptom score between 2 groups (P>0.05). After treatment,MMSE and MoCA score of 2 groups were increased significantly,while ADAS-Cog,ADL-R and TCM symptom score were decreased significantly;the treatment group were better than the control group,with statistical significance (P<0.05). No obvious ADR was found in 2 groups. CONCLU-SIONS:Congrong yishen granule combined with donepezil hydrochloride tablet can significantly improve mental state and cogni-tive ability of PDD patients,and relieve clinical symptoms with good safety.
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Objective To investigate the application of colposcope in cervical cancer screening in Yunnan ethnic minority areas.Methods The questionnaire survey was conducted on 641 women,and the data of the colposcopy and biopsy were reviewed retrospectively.The colposcopy and pathological diagnosis results were compared.Results The minority women thought colposcopy more acceptable was 78.64％ (383/487).The colposcopy found suspected low-grade cervical lesions were 377 cases,high-grade cervical lesions were 245 cases,cervical condyloma were 15 cases,cervical cancer were 4 cases.The pathological results:278 cases of cervical intraepithelial neoplasia (CIN) Ⅰ,174 cases of CIN Ⅱ,55 cases of CIN Ⅲ,15 cases of cervical condyloma,4 cases of cervical cancer.The consistent rate of colposcopy and pathological examination was 68.17％ (257/377) in low-grade lesions,89.39％ (219/245) in high-grade lesions,cervical cancer and cervical condyloma was entirely consistent.Conclusions The colposcopy is more easily accepted in Yunnan ethnic minority areas,which could be a preliminary diagnosis of cervical lesions,cervical condyloma and cervical cancer.It has an important clinical application value in diagnosis of cervical diseases.
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Malignant lymphoma is a common malignancy. From the perspective of the traditional Chinese medi-cine (TCM), the etiology and pathogenesis of malignant lymphoma are related to dampness, phlegm, toxicity, stasis and deficiency. Basic TCM principles for lymphoma syndrome differentiation and treatment are to strengthen the body resistance and eliminate pathogenic factors. Eliminating dampness, dissolving phlegm, detoxification, dissolv-ing stasis and strengthening body resistance are the principal TCM therapeutic methods for lymphoma treatment. Contemporary TCM physicians have developed Yi-Hua decoction, Zi-Niu powder, Ke-Liu capsule, Wu's Xiao-Liu powder and other herbal formulas, and 37 patents based on Chinese herbs and or herbal components. The effective mechanisms of TCM treatment for lymphoma are involved in inhibiting proliferation, inducing apoptosis, inhibiting angiogenesis, and regulating immune function. There is a great need to study anti-cancer herbs for lymphoma treat-ment.
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Intellectual property protection is an indispensable management measure in the process of knowledge innovation in the National Traditional Chinese Medicine (TCM) Clinical Research Bases. Longhua Hospital, a National TCM Clinical Research Base , has actively participated in studies and construction of TCM intellectual property policy , laws and regulations , and endeavored to enhance international research cooperation and broaden the international repercussions of TCM . Longhua Hospital made great efforts to strengthen management on intel-lectual property management team and measures, advocacy training, property rights formation and transactions. Intellectual property protection played an important role in the construction of the National TCM Clinical Re-search Base in Longhua Hospital .
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Cell senescence is an important anti-cancer mechanism and may contribute to cancer therapeutic outcome. The present study observed the effects of Tenglong Buzhong Decoction (TLBZD), a Chinese herbal formula, on senescence in human colon carcinoma LS-174-T cells.
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Objective: To observe the effects of Tenglong Buzhong Decoction (TLBZD), a compound traditional Chinese herbal medicine, on proliferation and apoptosis of colon carcinoma cell line LS174T in vitro. Methods: Human colon carcinoma cell line LS174T and human colon epithelial cell line CRL-1790 were treated with different doses of TLBZD. Cell proliferation was detected with cell counting kit-8 (CCK-8) assay and clone formation assay. Cell cycle and apoptosis were detected by flow cytometry, and caspase-3, -8 and -9 activities in LS174T cells were detected by colorimetric assay. Results: TLBZD had no obvious cytotoxicity in normal CRL-1790 cells. After 72-hour treatment of 1 mg/mL TLBZD, or 48- and 72-hour of 2 mg/mL TLBZD, or 24-, 48- and 72-hour of 5-20 mg/mL TLBZD, proliferation of LS174T cells was significantly inhibited. Clone formation of LS174T cells was significantly inhibited by 1 to 20 mg/mL TLBZD treatment. TLBZD at doses of 5 to 20 mg/mL also induced apoptosis and cell cycle arrest at G(0)/G(1) phase in LS174T cells. In addition, caspase-3, -8 and -9 activities were significantly elevated after 5 to 20 mg/mL TLBZD treatment. Conclusion: TLBZD can inhibit cell proliferation, arrest cell cycle at G(0)/G(1) phase, and induce apoptosis in LS174T cells, which may be related to activating of caspase-3, -8 and -9.
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Kidney theory is an important figure in traditional Chinese medicine (TCM), it proposes that the kidney is the root of life, in which stores congenital essence and energy of human body that promotes reproduction, growth and development. The facts that body development begins from oosperm have long been elucidated in modern medicine and life science. Furthermore, oosperm is a totipotent stem cell. The totipotent stem cell with its derived embryonic stem cell (ESC) and stem cell constitute the source of growth, development and tissue regeneration. It is rational to comprehend ESC by means of the Kidney theory of TCM, investigate the effect of Kidney-nourishing on the activities of ESC, such as proliferation, aging and apoptosis, and on its related genes regarding transcription, expression and functional regulation. Our works have suggested that classical Kidney-yin-nourishing herb formula Zuo-gui-wan could inhibit the ESC differentiation and apoptosis, stimulate proliferation, and promote the process of cell cycle. These observations coincide with up-regulate expression of Wnt and Oct3/4, and down-regulate expression of P16INK4a. At the same time, classical Kidney-yang-nourishing herb formula You-gui-wan could inhibit ESC apoptosis induced by H2O2, up-regulate Notch expression and down-regulate P16INK4a expression. In addition, there have been some evidences indicated Kidney-nourishing could promote mesenchymal stem cells proliferate. These observations strongly suggest that, Kidney theory of TCM, as the root of the life, is associated with ESC and stem cell; it may provide insight into Kidney theory of TCM and stem cell research.
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Objective To study the effects of tonifying kidney-yin on activities of embryo stem cell. Methods Embryonic stem cell line CRL-1825 was choosed as model cell in present study, and tonifying kidney-yin herb serum was added to CRL-1825 cell. The influence of tonifying kidney-yin on stem cell differentiation, cell cycle, apotosis and express of key genes were observed. Results Tonifying kidney-yin can inhibit stem cell differentiation and apotosis, promote stem cell proliferation and progression of cell cycle. In addition, tonifying kidney-yin can up-regulate Wnt, Oct4 gene expression, and down-regulation P16INK4a expression. Conclusions Tonifying kidney-yin can promote stem cell proliferation and maintain the state of stem cell.